Crystal Versus Solution Structures of Thiamine Diphosphate-dependent Enzymes
نویسندگان
چکیده
منابع مشابه
Crystal structures of phosphoketolase: thiamine diphosphate-dependent dehydration mechanism.
Thiamine diphosphate (ThDP)-dependent enzymes are ubiquitously present in all organisms and catalyze essential reactions in various metabolic pathways. ThDP-dependent phosphoketolase plays key roles in the central metabolism of heterofermentative bacteria and in the pentose catabolism of various microbes. In particular, bifidobacteria, representatives of beneficial commensal bacteria, have an e...
متن کاملDomain relationships in thiamine diphosphate-dependent enzymes.
Three-dimensional structures have been determined for 13 different enzymes that use thiamine diphosphate (ThDP) as a cofactor. These enzymes fall into five families, where members within a family have similar structures. In different families, there are similarities between some domains that clearly point to a common ancestor for all of these enzymes. Where the enzyme structures differ, evoluti...
متن کاملStudies on thiamine diphosphate-dependent enzymes.
The 3-deaza analogue of TPP (thiamine diphosphate), a close mimic of the ylid intermediate, has been synthesized and is an extremely potent inhibitor of a variety of TPP-dependent enzymes, binding much more tightly than TPP itself. Results using deazaTPP complexed with the E1 subunit of PDH (pyruvate dehydrogenase) have led to a novel proposal about the mechanism of this enzyme. The 2-substitut...
متن کاملEffects of thiamine deficiency and thiamine administration on the thiamine diphosphate-dependent enzymes in rat liver.
The activities of pyruvate and 2-oxoglutarate dehydrogenases were determined by a spectrophotometric method according to Massey, that of transketolase deter mined by Racker's method. The values of enzyme activity as well as the thiamine contents in the preparations were compared with those of the animals whose deficiencies were restored by thiamine administration. The thiamine deprivation resul...
متن کاملThiamine Diphosphate in Whole Blood, Thiamine and Thiamine Monophosphate in Breast-Milk in a Refugee Population
BACKGROUND The provision of high doses of thiamine may prevent thiamine deficiency in the post-partum period of displaced persons. METHODOLOGY/PRINCIPAL FINDINGS The study aimed to evaluate a supplementation regimen of thiamine mononitrate (100 mg daily) at the antenatal clinics in Maela refugee camp. Women were enrolled during antenatal care and followed after delivery. Samples were collecte...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.275.1.297